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anti s2 antibody  (Sino Biological)


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    Structured Review

    Sino Biological anti s2 antibody

    Anti S2 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d001+antibody/SARS-CoV-2+(2019-nCoV)+Spike+S2+Antibody%2C+Chimeric+MAb/med_rxiv__64898__2026__04__21__26351402-40-14-16
    Average 95 stars, based on 45 article reviews
    anti s2 antibody - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Diagnostic Classification for Long Covid Patients identifying Persistent Virus and Hyperimmune Pathophysiologies"

    Article Title: Diagnostic Classification for Long Covid Patients identifying Persistent Virus and Hyperimmune Pathophysiologies

    Journal: medRxiv

    doi: 10.64898/2026.04.21.26351402


    Figure Legend Snippet:

    Techniques Used:


    Figure Legend Snippet:

    Techniques Used: Diagnostic Assay

    Related Articles

    Expressing:

    Article Title: Reduced antibody cross-reactivity following infection with B.1.1.7 than with parental SARS-CoV-2 strains
    Article Snippet: Cells were incubated with sera (diluted 1:50 in PBS) for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide), and stained with BV421 anti-IgG (clone HP6017, Biolegend), APC anti- IgM (clone MHM-88, Biolegend), and PE anti-IgA (clone IS11-8E10, Miltenyi Biotech) for 30 min (all antibodies diluted 1:200 in FACS buffer). .. Expression of SARS-CoV-2 spike was confirmed by staining with the D001 antibody (40590-D001, SinoBiological). .. Cells were washed with FACS buffer and fixed for 20 min in CellFIX buffer (BD Bioscience).

    Article Title: Reduced antibody cross-reactivity following infection with B.1.1.7 than with parental SARS-CoV-2 strains
    Article Snippet: Cells were incubated with sera (diluted 1:50 in PBS) for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide), and stained with BV421 anti-IgG (clone HP6017, Biolegend), APC anti-IgM (clone MHM-88, Biolegend), and PE anti-IgA (clone IS11-8E10, Miltenyi Biotech) for 30 min (all antibodies diluted 1:200 in FACS buffer). .. Expression of SARS-CoV-2 spike was confirmed by staining with the D001 antibody (40590-D001, SinoBiological). .. Cells were washed with FACS buffer and fixed for 20 min in CellFIX buffer (BD Bioscience).

    Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases
    Article Snippet: SUP-T1 (CRL-1942) T cell lymphoblastic lymphoma cells were transduced with a pRV-based retroviral vector carrying a codon-optimized gene encoding the wild-type SARS-CoV-2 spike (UniProt ID: P0DTC2 ) and a gene encoding GFP, separated by an IRES. .. Expression of SARS-CoV-2 spike was detected by staining with the CR3022 antibody (Absolute Antibodies) or D001 antibody (40590-D001, SinoBiological). ..

    Staining:

    Article Title: Reduced antibody cross-reactivity following infection with B.1.1.7 than with parental SARS-CoV-2 strains
    Article Snippet: Cells were incubated with sera (diluted 1:50 in PBS) for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide), and stained with BV421 anti-IgG (clone HP6017, Biolegend), APC anti- IgM (clone MHM-88, Biolegend), and PE anti-IgA (clone IS11-8E10, Miltenyi Biotech) for 30 min (all antibodies diluted 1:200 in FACS buffer). .. Expression of SARS-CoV-2 spike was confirmed by staining with the D001 antibody (40590-D001, SinoBiological). .. Cells were washed with FACS buffer and fixed for 20 min in CellFIX buffer (BD Bioscience).

    Article Title: Reduced antibody cross-reactivity following infection with B.1.1.7 than with parental SARS-CoV-2 strains
    Article Snippet: Cells were incubated with sera (diluted 1:50 in PBS) for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide), and stained with BV421 anti-IgG (clone HP6017, Biolegend), APC anti-IgM (clone MHM-88, Biolegend), and PE anti-IgA (clone IS11-8E10, Miltenyi Biotech) for 30 min (all antibodies diluted 1:200 in FACS buffer). .. Expression of SARS-CoV-2 spike was confirmed by staining with the D001 antibody (40590-D001, SinoBiological). .. Cells were washed with FACS buffer and fixed for 20 min in CellFIX buffer (BD Bioscience).

    Article Title: SARS-CoV-2 mRNA-based vaccines in the Aicardi Goutières Syndrome.
    Article Snippet: .. Transfected cells were harvested 48 h posttransfection, washed once, resuspended in FACS buffer (1× PBS, 2% FBS, 0.05% sodium azide) at 1 × 106 cell/mL, and stained with 10 μg/mL D001 antibody (Sino Biological) in U-bottom 96-well plates for 30 min at 4 °C. .. Cells were washed once and incubated with a PElabelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12–4998-92) at a final concentration of 2.5 μg/mL of 30min at 4 °C.

    Article Title: mRNA-based vaccines against SARS-CoV-2 do not stimulate interferon stimulatory gene expression in individuals affected by Aicardi Goutières Syndrome
    Article Snippet: .. Transfected cells were harvested 48 hours post-transfection, washed once, resuspended in FACS buffer (1X PBS, 2% FBS, 0.05% sodium azide) at 1×10 6 cell/ml, and stained with 10 μ g/mL D001 antibody (SinoBiological) in U-bottom 96 well plates for 30 minutes at 4°C. .. Cells were washed once and incubated with a PE-labelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12-4998-92) at a final concentration of 2.5 μ g/mL of 30 minutes at 4°C.

    Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases
    Article Snippet: SUP-T1 (CRL-1942) T cell lymphoblastic lymphoma cells were transduced with a pRV-based retroviral vector carrying a codon-optimized gene encoding the wild-type SARS-CoV-2 spike (UniProt ID: P0DTC2 ) and a gene encoding GFP, separated by an IRES. .. Expression of SARS-CoV-2 spike was detected by staining with the CR3022 antibody (Absolute Antibodies) or D001 antibody (40590-D001, SinoBiological). ..

    Transfection:

    Article Title: SARS-CoV-2 mRNA-based vaccines in the Aicardi Goutières Syndrome.
    Article Snippet: .. Transfected cells were harvested 48 h posttransfection, washed once, resuspended in FACS buffer (1× PBS, 2% FBS, 0.05% sodium azide) at 1 × 106 cell/mL, and stained with 10 μg/mL D001 antibody (Sino Biological) in U-bottom 96-well plates for 30 min at 4 °C. .. Cells were washed once and incubated with a PElabelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12–4998-92) at a final concentration of 2.5 μg/mL of 30min at 4 °C.

    Article Title: mRNA-based vaccines against SARS-CoV-2 do not stimulate interferon stimulatory gene expression in individuals affected by Aicardi Goutières Syndrome
    Article Snippet: .. Transfected cells were harvested 48 hours post-transfection, washed once, resuspended in FACS buffer (1X PBS, 2% FBS, 0.05% sodium azide) at 1×10 6 cell/ml, and stained with 10 μ g/mL D001 antibody (SinoBiological) in U-bottom 96 well plates for 30 minutes at 4°C. .. Cells were washed once and incubated with a PE-labelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12-4998-92) at a final concentration of 2.5 μ g/mL of 30 minutes at 4°C.

    FACS:

    Article Title: SARS-CoV-2 mRNA-based vaccines in the Aicardi Goutières Syndrome.
    Article Snippet: .. Transfected cells were harvested 48 h posttransfection, washed once, resuspended in FACS buffer (1× PBS, 2% FBS, 0.05% sodium azide) at 1 × 106 cell/mL, and stained with 10 μg/mL D001 antibody (Sino Biological) in U-bottom 96-well plates for 30 min at 4 °C. .. Cells were washed once and incubated with a PElabelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12–4998-92) at a final concentration of 2.5 μg/mL of 30min at 4 °C.

    Article Title: mRNA-based vaccines against SARS-CoV-2 do not stimulate interferon stimulatory gene expression in individuals affected by Aicardi Goutières Syndrome
    Article Snippet: .. Transfected cells were harvested 48 hours post-transfection, washed once, resuspended in FACS buffer (1X PBS, 2% FBS, 0.05% sodium azide) at 1×10 6 cell/ml, and stained with 10 μ g/mL D001 antibody (SinoBiological) in U-bottom 96 well plates for 30 minutes at 4°C. .. Cells were washed once and incubated with a PE-labelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12-4998-92) at a final concentration of 2.5 μ g/mL of 30 minutes at 4°C.



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    Image Search Results


    Journal: medRxiv

    Article Title: Diagnostic Classification for Long Covid Patients identifying Persistent Virus and Hyperimmune Pathophysiologies

    doi: 10.64898/2026.04.21.26351402

    Figure Lengend Snippet:

    Article Snippet: The integrity of the spike protein samples on the surface was tested using an anti-S2 antibody (SinoBiological, 40590-D001), chosen for its specificity to a pan-variant conserved epitope.

    Techniques:

    Mean-normalised concentration data for CC (blue) and LC (red). All variants are considered together for this analysis. Significant differences are present between the cohort medians for IgG1, IgG3 and IgG4 using a Mann Whitney U test with Holm-Sidak correction (Data shown in ). (*) indicates significance p<0.05, (**) indicates significance p<0.005. Some outliers for the IgG1 control data lie outside of the plotted y-values and can be found in

    Journal: medRxiv

    Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

    doi: 10.64898/2026.01.26.26344863

    Figure Lengend Snippet: Mean-normalised concentration data for CC (blue) and LC (red). All variants are considered together for this analysis. Significant differences are present between the cohort medians for IgG1, IgG3 and IgG4 using a Mann Whitney U test with Holm-Sidak correction (Data shown in ). (*) indicates significance p<0.05, (**) indicates significance p<0.005. Some outliers for the IgG1 control data lie outside of the plotted y-values and can be found in

    Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

    Techniques: Concentration Assay, MANN-WHITNEY, Control

    Single patient results showing the variant specific response to each IgG subclass measured separately for IgG1-IgE but for all variants simultaneously: A) Control sample and B) Long Covid sample: IgG Subclass concentrations IgG1-4: coloured as pink (IgG1), blue (IgG2), yellow (IgG3), green (IgG4) and grey (IgE).

    Journal: medRxiv

    Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

    doi: 10.64898/2026.01.26.26344863

    Figure Lengend Snippet: Single patient results showing the variant specific response to each IgG subclass measured separately for IgG1-IgE but for all variants simultaneously: A) Control sample and B) Long Covid sample: IgG Subclass concentrations IgG1-4: coloured as pink (IgG1), blue (IgG2), yellow (IgG3), green (IgG4) and grey (IgE).

    Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

    Techniques: Variant Assay, Control

    Antibody IgG subclass and IgE concentration distributions presented by SARS-CoV-2 Spike protein variant: CC (blue) and LC (red) cohorts. (A) IgG1; (B) IgG2, (C) IgG3, (D) IgG4 and (E) IgE. The boxplots show the medians and interquartile range. The vertical lines on each box point to the extremes of the distributions. Significant results to the p< 0.05 (*) and p<0.005 (**) level determined by the Mann-Whitney U test with Holm-Sidak correction applied

    Journal: medRxiv

    Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

    doi: 10.64898/2026.01.26.26344863

    Figure Lengend Snippet: Antibody IgG subclass and IgE concentration distributions presented by SARS-CoV-2 Spike protein variant: CC (blue) and LC (red) cohorts. (A) IgG1; (B) IgG2, (C) IgG3, (D) IgG4 and (E) IgE. The boxplots show the medians and interquartile range. The vertical lines on each box point to the extremes of the distributions. Significant results to the p< 0.05 (*) and p<0.005 (**) level determined by the Mann-Whitney U test with Holm-Sidak correction applied

    Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

    Techniques: Concentration Assay, Variant Assay, MANN-WHITNEY

    Correlation between Total IgG and subclass measurements: A) Control Cohort, B) Long Covid Cohort. Marker shape denotes subclass: IgG1 (o), IgG2 (x), IgG3 (□), IgG4 (◊) and IgE (Δ). Regression lines are ordered vertically and IgG1 and IgG4 production dominates in long COVID patients compared with IgG1 and IgG2 in controls; A) controls IgG1-IgG2-IgG3-IgG4-IgE; and B) long COVID IgG1-IgG4-IgG2-IgE-IgG3.

    Journal: medRxiv

    Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

    doi: 10.64898/2026.01.26.26344863

    Figure Lengend Snippet: Correlation between Total IgG and subclass measurements: A) Control Cohort, B) Long Covid Cohort. Marker shape denotes subclass: IgG1 (o), IgG2 (x), IgG3 (□), IgG4 (◊) and IgE (Δ). Regression lines are ordered vertically and IgG1 and IgG4 production dominates in long COVID patients compared with IgG1 and IgG2 in controls; A) controls IgG1-IgG2-IgG3-IgG4-IgE; and B) long COVID IgG1-IgG4-IgG2-IgE-IgG3.

    Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

    Techniques: Control, Marker

    Journal: medRxiv

    Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

    doi: 10.64898/2026.01.26.26344863

    Figure Lengend Snippet:

    Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

    Techniques: Transformation Assay

    The variant profiles showing the long COVID classification triggers: (A) IgG1 below threshold, T; and (B) IgG3 (C) IgG4 (D) IgE above threshold, T, derived from the percentile (T) in the mean-normalised variant concentration distributions in the Control Cohort.

    Journal: medRxiv

    Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

    doi: 10.64898/2026.01.26.26344863

    Figure Lengend Snippet: The variant profiles showing the long COVID classification triggers: (A) IgG1 below threshold, T; and (B) IgG3 (C) IgG4 (D) IgE above threshold, T, derived from the percentile (T) in the mean-normalised variant concentration distributions in the Control Cohort.

    Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

    Techniques: Variant Assay, Derivative Assay, Concentration Assay, Control